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Few cells labeled for activated caspase-3 are present in the E14.5 intestine, but the marker of apoptosis is not found inside the vasculature or in GFP + cells (Supplementary Fig

Then, 20 L of MTT solutions was added to each well followed by an incubation period of 3 hours, then the media were removed and 200 l of DMSO was added to dissolve the crystal and the absorbance was recorded at 570 nm (BioTek, USA) and cell viability was calculated as a percentage compared to cells treated with DMEM alone

Hydroxyl radicals ( OH) and oxidative damage Hydroxyl radicals ( OH) are the most reactive species among ROS and can cause severe oxidative damage in biological systems

J Liposome Res 35, 153158.CrossRefGoogle Scholar Byeon, JC, Lee, SE, Kim, TH, et al