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Mitochondrial suspension was added to the experimental chamber to achieve a final protein concentration of 2.52.7 mg/ml

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Across human macrophage models and rodent LPS-challenge systems, sulforaphane-mediated Nrf2 activation reduces intracellular reactive oxygen species, attenuates NF-B signaling, and lowers pro-inflammatory cytokine release, including TNF- and IL-6 (Yan and Yan, 2023