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Description
T-cells become activated when they are exposed to antigen (Steinman, 1991
The fixed monolayers were permeabilized and blocked with 100 L/well 0.5% Triton X-100, 5% normal donkey serum in PBS in the apical chamber for 1 h at RT, then incubated with anti-MUC2 antibody clone CCP58 (1:100 dilution
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