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Cross-linking assay of Sm TG For the BSA cross-linking assay of Sm TG at different reaction times, 40 L of the reaction buffer BD [1.25 mg/mL BSA, 50 M dithiothreitol (DTT)] and 10 L of the 1 Sm TG solution in 100 mM TrisHCl (pH 8.0) were mixed and incubated for 0, 30, 60 and 120 min at 50 C

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Journal of proteome research , 24 (2), 537549

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