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In addition, a clear boundary between tumor and normal tissue was observed
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On the subsequent days cells were washed in DMEM/F12 medium (Corning, USA) and the medium was changed, as follows: Basal medium: S1 MCDB131 (Corning, USA), 1% Glutamax (Thermo Fisher Scientific, Netherlands) + 1% Pen/Strep (Thermo Fisher Scientific, Netherlands), 8.88 mM glucose (Merck Millipore, USA), 14 mM sodium bicarbonate (Merck Millipore, USA) and 0.1% BSA (Capricorn Scientific, USA)
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