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Supplementary Material 2: Additional file 2.pdf contains the figure: Immunohistofluorescence analysis of claudin-1 and claudin-2 localization in the brain of control, vehicle-treated/CDNB perfused and DMF-treated/CDNB perfused 3-day-old rats

The biological activity of Se varies depending on its concentration: normal growth and development at low concentrations, homeostatic function at moderate concentrations, and induced toxicity at high concentrations [95]

Once positive expression is confirmed, DNA sequencing should be performed to ensure no errors were introduced during the PCR amplification or ligation
