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Briefly, 10 mg of tissue was lysed in 1 mL of Lysis buffer, and nuclei were extracted using a 1 mL dounce homogenizer

To dissect the link between MG detoxification and intracellular fitness, macrophages were pre-treated with 0.1 mM MG for 4 h to mimic a carbonylstressed host environment ( Figure 4A )

In all cases these effects occurred within a very narrow dose range governed by the reactivity, volatility, and cytotoxicity of acrolein

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