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Hence, we first introduced DPPC liposomes into the measurement chamber for 4 min to obtain an increasing peak shift value, indicating that DPPC liposomes were adsorbing onto the sensor surface
To corroborate these findings, a UV-vis analysis was performed by adding Cu(NO 3 ) 2 to a fixed, 100 M solution of TC-Py and monitoring the change in absorbance between 270 and 800 nm (Supplementary Fig
Prior to the analytical SEC runs the column was equilibrated with SEC buffer (25 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM -mercaptoethanol) and protein concentrations adjusted to 1.02.0 mg/mL in same buffer in a final applied volume of 100 L

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