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glutathione agarose affinity chromatography principle An alternative easy method for antibody purification and analysis of protein–protein interaction using GST fusion proteins immobilized onto glutathione–agarose | Analytical and Bioanalytical Chemistry 1 and 3ml with total column volumes of 1 Affinity Chromatography: Principle, Parts, Steps,
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Since this study focuses on wound-associated pain, a biochemical approach, such as quantifying levels of pain mediators (e.g., substance P) in body fluids at defined time points, appears to be the most desirable method

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