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Early biochemical changes occur, such as an increase in homocysteine and methylmalonic acid (MMA), which indicate impaired B12 function
Captured mAb variants were detected by an APconjugated polyclonal anti-human IgG Fc specific Ab from goat (Sigma-Aldrich, A9544) (diluted 1:5000 in PBS/T/S) and visualized by addition of p-nitrophenylphosphate (Sigma-Aldrich) (10 g/mL in diethanolamine buffer)
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doi: 10.1007/s11157-016-9390-1 44 HafeezB.KhanifY.SaleemM
