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Although the application of shotgun in nematode lipid studies is unusual, it is agreed that shotgun lipidomics will be widely used due to its attractive advantages such as the simplicity of the process, high resolution, and automation of the sample-related operations

Tissue samples were subjected to total RNA isolation with the RNeasy Mini Kit (Qiagen Cat

doi: 10.3389/fphar.2025.1591825 Received 11 March 2025 Accepted 28 August 2025 Published 08 October 2025 Volume 16 - 2025 Edited by Er Sheng Gong, Gannan Medical University, China Reviewed by Guillermo Federico Spitzmaul, National Scientific and Technical Research Council (CONICET), Argentina Leonardo Dionisio, National Scientific and Technical Research Council (CONICET), Argentina Updates Copyright 2025 Giannini, Soldi, Elli, Sagheddu, Castagnetti, Viciani, Salvini, Battistuzzi, Milazzo, Alibrandi and Sidoti