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In the liver of db/db mice and HG-induced HepG2 cells, ferroptosis was observed, characterized by iron accumulation accompanied by decreased SLC7A11 expression, increased activities of SOD, GSH-PX, and GSH, as well as elevated levels of MDA, 4-HNE, NOX4, and ROS [153]

However, the authors also stressed the need for larger and more definitive trials before firm clinical conclusions can be drawn [184]

The individual peaks of the identified metabolites were compared with their corresponding spectrum via the NIST library, a public database

Cells were harvested at 12,000 x g for 15 min at 18 C and stored at 20 C until purification