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After 48 h incubation of confluent monolayers with cARLA, the culture medium was removed and hECs were fixed with a 1:1 mixture of ice cold methanol-acetone solution for 2 min
While its relatively easier to be in a caloric deficit when fasting, false assumptions that intermittent fasting alone results in weight loss may lead to overconsumption during non-fasting periods, and hence unsatisfactory outcomes
Therefore, we recommend clinicians against relying solely on BMI to evaluate sarcopenia in liver cirrhosis
Liver and renal markers, as well as other parameters, were all within normal limits
