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Description
The trial was conducted thrice, and the viability of cells was determined by applying the formula: Cell viability = (average absorbance of experimental wellsaverage absorbance of control wells with medium) / (average absorbance of untreated wellsaverage absorbance of control wells with medium) 100%
Peptide-based supramolecular assembly drugs toward cancer theranostics

For each compound, the Log2 fold change (Log2 FC) of the mean pool size ( n = 3) in the experimental condition (AMW25, AMW30, AMW35, AMW40, AMW45, AMW50, AMW55, AMW60) was calculated with respect to the control condition (AMW20)

Compared with 2D monolayer culture, 3D culture exhibits remarkable tumorigenicity, better simulating in vivo behavior, facilitating mechanism research, and aiding in the development of targeted drugs (Antoni et al., 2015)