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Higgins JPT, Thomas J, Chandler J et al (2021) Cochrane handbook for systematic reviews of interventions version 6.2 Sterne JAC, Savovi J, Page MJ et al (2019) RoB 2: a revised tool for assessing risk of bias in randomized trials

No, said Julie Rhee, a Korean American pharmacist with a doctorate in pharmaceutical studies

It confirmed ferroptosis as the core mechanism in PD pathogenesis synergistically induced by iron overload and inflammation, which activate ferroptosis by disrupting iron metabolism, inhibiting the antioxidant system and exacerbating lipid peroxidation, further causing dopaminergic neuronal damage, -syn aggregation, and PD-characteristic motor/cognitive impairments (186).Treating SH-SY5Y dopamine neurons with rotenone and using RhoNox-1 staining, followed by confocal microscopy observation and flow cytometry analysis, revealed significantly higher intracellular Fe 2+ in the rotenone group, confirming cellular iron overload (187)
