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KEAP1-independent Nrf2 degradation (e.g., by glycogen synthase kinase-3 (GSK-3) is regulated by the Neh6 domain [293]
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Immunofluorescence in vitro To detect the change of HIF-1 expression, A375 cells were seeded at a density of 5 10 3 cells/well for 24 h under hypoxic (1% O 2 , 5% CO 2 , and 94% N 2 ) and normoxic (21% O 2 , 5% CO 2 , and 74% N 2 ) conditions, respectively, and then incubated with blank medium, 5-ALA, CCPA, and CCPCA NPs for another 24 h
