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These requirements of man for methionine, and the sparing effects of cysteine, determined in young healthy volunteers in 1955 by Rose et al are still accepted today, in spite of indications that they may not represent universal values [5]

Depending on the nature of the samples, MALDI-MS analysis can be performed in three primary modes: direct sample analysis, in situ tissue detection, and tissue imaging

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Cascone T, Sepesi B, Lin H, Kalhor N, Parra ER, Jiang M, Godoy MCB, Zhang J, Fossella F, Tsao AS, Lam VK, Lu C, Mott FE, Simon GR, Antonoff MB, Reza MJ, Rice D, Behren C, Weissferdt A, Moran C, Vaporciyan AA, Lee JJ, Swisher SG, Gibbons DL, Wistuba II, William WN, Heymach JV