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Description
For invasion assays, 1 10 5 cells in 200 L of serum-free media were seeded in the upper chamber of an insert coated with Matrigel (BD Biosciences, San Diego, CA)
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It is known that hydrogen can be applied in various forms (hydrogen gas, hydrogen-rich water, hydrogen-rich nano-bubbled water, magnesium hydride, etc.) (Table 7)
Some individuals may experience mild reactions, such as headaches, nausea, or a feeling of warmth
