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Procedure includes deeply anesthetized rats, prone position, cleaned operative field, median incision, exposing the retroperitoneum, identifying the structures, preparation of the aorta, placing the clamps, horizontal cut between renal arteries and bifurcation (common illiac arteries) and termino-terminal anastomosis creation (8/0 Prolene sutures) ( i.e

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8 A case-control study that compared 25,956 patients who had vitamin B 12 deficiency with 184,199 control patients found a significantly increased risk of vitamin B 12 deficiency in patients who had taken proton pump inhibitors (odds ratio = 1.65) or histamine H 2 blockers (odds ratio = 1.25) for at least two years