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Description

Abbreviations CTD C-terminal domain DAG diacylglycerol ET-18-OCH3 1 - 0 -octadecyl- 2 - 0 -methyl-glycerol-3-phosphocholine GAP GTPase activating protein GPCR G proteincoupled receptor IP 3 inositol-1,4,5-triphosphate PH pleckstrin homology PIP 2 phosphatidylinositol-4,5-bisphosphate PLC phospholipase C TIM triose phosphate isomerase U73122 1-(6-((17 -3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1 H -pyrrole-2,5-dione Authorship Contributions Wrote or contributed to the writing of the manuscript : Lyon, Tesmer

doi: 10.1038/ajh.2010.142

We have made a number of changes to existing protocols, most notably, the use of defined salt concentrations (0.175 M NaCl) in the working buffer, the use of an ApoC-II peptide as a potent, readily available and cost-effective LPL activator, and the use of the nAngptl4 peptide as an LPL-specific inhibitor