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However, peptide-based research has long been constrained by physicochemical challengesnamely, enzymatic degradation, limited membrane permeability, and rapid systemic clearance

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Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma #IPVH00010), blocked for an hour using 5% milk in TBST, and stained overnight with targeting primary antibodies in 5% milk TBST

This system regulates disulfide bond formation, redox-sensitive transcription factors, and apoptosis