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In addition to normal digestive system-related excretion, production animals excrete zinc with their products

In hypertensive states, mitochondria generate excessive ROS due to electron transport chain (ETC) disruption, particularly at complexes I and III, coupled with reduced antioxidant defenses (e.g., Mn-SOD) (Grossini et al., 2025)

After incubation, the dye was removed, and the DhLOs were gently washed twice with cold PBS containing calcium and magnesium (Gibco, Thermo Fisher Scientific, #10010023)
However, it is a challenge to determine -Syn in CSF, depending on careful sample collection and processing