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A single high-protein meal containing 23 g tryptophan can transiently elevate serum IS by 30%50% within 612 h, with fasting status, protein source (animal vs
Lee JW, Aminkeng F, Bhavsar AP, et al
If you are working with a poor antibody or a very low-abundance protein, you could try to increase binding by incubating overnight
[DOI] [PMC free article] [PubMed] [Google Scholar] Jiang H, Ren Y, Zhao J, Feng J
