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5A-1MQ-treated mice switched from WD to LD (WD/LD-T) showed a rather reduced relative abundance of Akkermansia compared to vehicle-treated DIO animals switched from WD to LD (WD/LD-V), suggesting a unique microbiome mechanism associated with weight loss in the vehicle-treated group switched from WD to LD compared to the 5A-1MQ-treated group that underwent the same diet switch

Although dietary fiber has been associated with microbiome changes 75 , this is unlikely to account for the microbiome changes observed herein since fiber content was the same across diets

If single morning administration produces satisfactory results, adding complexity provides no advantage