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Approximately 18-24 h after transfection, the culture medium was changed to low-inositol Hams F-10 medium (Gibco) containing 1.5 Ci/well Myo-[2- 3 H(N)]inositol (Perkin Elmer) for 16-18h

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The prevailing theory prior to this structure was that an open lipase conformation was defined by a displaced lid peptide, exposing the hydrophobic pocket surrounding the active site

Following the breakthrough discovery that small RNA-based pathways emerge as regulatory mechanisms for post-transcriptional control of gene expression in multicellular eukaryotes 19,20 , these RNA species have gained significant attention