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In this investigation, we initially identified oridonin as a direct covalent inhibitor of GSH, setting it apart from other drugs that disrupt ROS by targeting proteins both upstream and downstream of GSH

The frozen-thawed sperm viability was analyzed by eosin-nigrosin staining (55, 58)
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A blood sample was obtained at this visit to assess reduced, oxidized and total glutathione as oxidative stress biomarkers