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fowleri Cells The grown cells were washed twice in S-M buffer (250 mM Saccharose, 10 mM MOPS, pH 7.2) and disrupted by sonication using SONOPULS ultrasonic homogenizer mini20 (BANDELIN, Germany) with the following settings: amplitude 30%, 1/1 s pulse for 1 min on ice

Together, they form a powerhouse treatment that helps your body rebuild from the inside out

Acta Neuropsychiatr 2018;30:334-41

So it would be possible to have a normal or even high level of serum B12, and have most of that be inactive, and still be suffering from B12 deficiency, because you dont really have enough of the active B12 that gets in the cells