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In general, it is assumed that the two main limiting factors are the levels of cysteine and of the enzyme gamma-glutamyl-cysteine synthetase (also known as glutamate-cysteine ligase)
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c) leaves, 20 min in a vacuum chamber with 50 mM DTT or 50 mM H 2 O 2
and from a nosy baby sister who was always leaning on me