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Cell death was shown by the change in cell viability, which was calculated as: (viable cell)% = (optical density (OD) of untreated sample/OD of treated sample) 100

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Allergy management reduces chronic periorbital inflammation driving melanin production, vascular dilation, and fluid retention - users should identify and address environmental allergies through appropriate medications or avoidance, manage seasonal allergies proactively before symptoms worsen, avoid rubbing eyes despite itching creating mechanical irritation and post-inflammatory hyperpigmentation, and consider antihistamine eye drops for direct ocular symptom relief

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