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doi:10.1002/cld.865 [PubMed] 115

Total RNA was isolated using the RNeasy Mini Kit (Qiagen) and equal amounts of all samples were reverse transcribed with SuperScript III (Invitrogen) using oligo(dT)1218 primers

The cells incubated with control medium were considered 100% viable

Human NK cells directly recognize Mycobacterium bovis via TLR2 and acquire the ability to kill monocyte-derived DC
