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In addition, we use filter-tips or filter plates to remove the flow-through after binding the phosphopeptides to the beads, to wash the beads, and elute the phosphopeptides using centrifugation or a vacuum manifold, respectively
doi:10.1056/nejmcp1106637
Why Cycling Matters Receptor sensitivity : Taking breaks helps prevent receptor downregulation, maintaining the peptide's effectiveness over time
2012 Dec;59(3):673-8
