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Do not massage the site vigorously

For each analysis, 100 L of KH-GSNO stock solutions (20 mM or 50 mM) were injected into the reaction chamber containing 10 mL of KH solution (pH 7.4), maintained at 37 C and protected from light, resulting in final GSNO concentrations in the chamber of 200 M and 500 M, respectively

Rupesh K S (2015) Probiotics and bone health: it takes guts to improve bone density

Burrell et al., 2016)