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Description
Navarro J, Morafernandez C
(Modified from Barrett KE: Gastrointestinal Physiology
This remarkable range of motion comes at a cost: vulnerability to injury and notoriously slow healing times
Load a half-area, black, round-bottom, polystyrene, 96-well plate with duplicate 50 l samples of the following in Ca 2+ -free CLM: Serially diluted competing ligand (e.g., IP 3 , final concentrations 3 nM-150 M), FITC-IP 3 (final concentration 0.5 nM) and NT (from Step 40, final concentration 80 nM)
