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Ice-crystal formation during each freeze-thaw cycle inflicts mechanical damage on peptide chains, and pre-splitting eliminates the cumulative loss that comes from thawing one vial multiple times

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For comparisons between two specific groups, the MannWhitney U test was applied

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Food and Drug Administration (2024) FDAs concerns with unapproved GLP-1 drugs used for weight loss, U.S

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As a result, it should be used with caution

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