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After drying, slides were mounted with ProLong Gold (Thermo Fisher Scientific) and visualized using confocal microscopy (LSM900 with Airyscan
Bait proteins can be expressed in bacterial, mammalian, insect, or cell-free systems, while prey proteins can come from cell lysates, recombinant expression, or purified protein preparations
For significant fading of dark spots or tan removal, it generally takes 8 to 12 weeks to see full effects following a complete skin cell turnover
