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In this case, the movement of a molecule across membranes does not require energy, and the diffusion process finishes when the concentration of the molecule in both sides of the membrane attains equilibrium, driven by the potential energy difference of a concentration gradient

Rabbit anti-human GSTM1 antibody (Upstate Biotechnology, Lake Placid, NY, USA), rabbit anti-human GSTM2 antibody (Lifespan Biosciences, Inc, Seattle, WA) and rabbit anti-human beta-actin antibody (Santa Cruz Biotechnologies, CA, USA) were used in 1:1000 dilutions, followed by goat anti-rabbit IgG (GE Healthcare) as the secondary antibody (1:2000 dilutions)

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Additionally, in SH-SY5Y cells, melatonin increased levels of Peptidyl-Prolyl cis-trans Isomerase NIMA-Interacting 1 (Pin1) [125], which has been shown to mitigate tau phosphorylation and aggregation [126]