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Mitochondrial ironsulfur protein biogenesis and human disease

Then, the medium was removed and the cells were fixed with 4% paraformaldehyde for 10 min at room temperature, followed by incubation with 5 g/mL Hoechst 33258 (Invitrogen, Carlsbad, CA, USA) for 10 min at 37 2 C in a 5% CO 2 -humidified incubator

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Given that A42 peptide oligomers are resident in the lipid bilayer (17, 18, 21, 120), coupled with the requirement that a lipid-resident free radical is necessary to initiate the processes of lipid peroxidation (free radicals outside the bilayer would be too reactive to survive entering the bilayer in the first place), consistent with the location of the Met35 residue of A42, we posit that the sulfuranyl free radical of A42 is the initiator of lipid peroxidation associated with this peptide (FIGURE 8)