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Description
Copper can enhance expression of MMP-1, an enzyme that breaks down collagen
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Before use, the protein was denatured in 6 M urea and supplemented with 2 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, Solna, Sweden) at pH 7.4 to obtain complete reduction and dissolution of potential aggregates
Ribeiro JA, dos Santos Pereira E, de Oliveira Raphaelli C, Radnz M, Camargo TM, da Rocha Conceno FIG et al (2022) Application of prebiotics in apple products and potential health benefits
