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Main advantages of magnetic beads for biotin-binding: If your target protein or ligand needs to be covalently coupled, use surface-activated beads Different ligands (antibodies, proteins, etc.) require different bead surface properties and immobilization chemistries

For example, in a typical 96-well plate format for an equilibrium competition FP binding assay, we obtain K D values for 3 different ligands from a single plate (with duplicate determinations of 15 concentrations for each ligand) at 6 different temperatures in less than 3 h

& Windsor, J
