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This allows for a sufficient trial length and will allow for enough B12 to make a difference over a sustained period of time

In the liver, the 25-hydroxylase enzymes (CYP2R1 and CYP27A1) convert inactive cholecalciferol to 25(OH)VD 3 (calcidiol) by carbon 25 hydroxylation 14

The optical density was measured at 412 nm without delay, and the results were reported as micromoles of GSH per milligram of protein
