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Description
Cells were washed three times with ice-cold PBS and lysed in lysis buffer (40 mM Tris-HCl (pH 8.0), 1% Triton X-100, 1% NP-40, 10% glycerol, 0.15 M NaCl, 2 mM EDTA, 1 mM phenylmethanesulfonyl fluoride, 1 Complete protease inhibitor cocktail)
Initially, the PBS, CaT-ME, and CaT groups were evaluated via skin penetration experiments using FITC-labeled copper peptides (Fig

This is because the tissues that form the abdominal wall dont regenerate in a way that would close the defect

Human evidence remains limited and exploratory
