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These receptors are highly promising targets for obesity treatment because of their vital functions and relative specificity in energy homeostasis [55]

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For the GSH assay, the lungs were harvested, washed in PBS, and then homogenated in 0.1 M phosphate buffer containing 5 mM EDTA, 0.1 % (vol/vol) Triton X-100, and 0.6 % (wt/vol) sulfosalicylic acid

They found that it wasnt possible to determine a clear lower limit for which any of these biomarkers can reliably indicate B12 deficiency, but that daily intakes above 4.0 g appear to have no additional benefit