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In another set of experiments, cells were preincubated with 10 M LY294002 for 30 min at 37 C and then stimulated with bTNF- for 5 min
Lower doses may be insufficient to generate meaningful microbiota changes, while excessive intake can cause gastrointestinal discomfort
DNA was eluted in 100 L of preheated NE buffer and quantified
S.et al (1998)
