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Description
The difference between doing it right and doing it wrong comes down to three things most protocols never mention: pre-reconstitution freezer storage at 20C, strict 28C refrigeration post-mixing, and the 28-day viability window that begins the moment bacteriostatic water contacts the lyophilised powder

& Withers, D
10.1155/2015/764641 152 TakahashiK.TanabeK.OhnukiM.NaritaM.IchisakaT.TomodaK.et al (2007)

Blood from the trunk was collected and were treated the same as previously described 33 till we get the homogenized tissue and stored at 80 C until using
