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As a proxy for neuronal activity, neurons were exposed to anti-Syt1 lumenal primary antibody directly labeled with ATTO647N (1:200, 105 311AT1 clone 604.2 Synaptic Systems) in conditioned medium for 30 mins at 37 C

Curr Med Sci 40:1827 Mori T, Koyama N, Tan J, Segawa T, Maeda M, Town T (2019) Combined treatment with the phenolics ()-epigallocatechin-3-gallate and ferulic acid improves cognition and reduces Alzheimer-like pathology in mice

Metformin (N,N-dimethylbiguanide), a standard clinical drug for type 2 diabetes mellitus (T2DM) for over 60 years (Foretz et al., 2023), has been shown to have multiple biological effects beyond its hypoglycemic properties, such as antioxidant, anti-inflammatory, anti-tumor, anti-fibrotic, and antiviral activities (Petrasca et al., 2023

Phosphorylation of these sites was later shown to occur through the distinct activities of ARK, PKA and PKC [15]