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Biotinylated proteins eluted from the z13 beads were detected by SDSpolyacrylamide gel electrophoresis (PAGE) followed by peroxidase avidin blot and an ECL (Amersham, Piscataway, NJ) chemiluminescence reaction
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In Vitro Studies In vitro studies have demonstrated that Epithalon can activate telomerase in cultured human cells, including fibroblasts and lymphocytes, with measurements showing increased telomerase activity by TRAP assay and subsequent telomere lengthening
