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Metabolomic analysis We used Biocrates AbsoluteIDQ p180 kit to analyze cardiac metabolites from female mice [Young ( n = 7), Old Con ( n = 8), and Old mAb ( n = 7)] with UPLC-MS/MS Xevo TQ, Waters, Pittsburgh, PA, USA) in the Einstein Stable Isotope and Metabolomics Core, according to the manufacturers instructions (BIOCRATES Life Sciences AG, Innsbruck, Austria)

This ongoing research is crucial for understanding how these compounds can be safely and effectively utilized in clinical practice

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