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Intracellular concentration of glutathione was measured using the GSH-Glo(tm) Glutathione Assay from Promega
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Tamoxifen increases the levels of ceramide, JNK phosphorylation and markers of apoptosis We next determined the effects of tamoxifen on caspase-3 activation, which is known to cleave poly (ADP-ribose) polymerase (PARP) and other proteins leading to apoptosis
