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A genome-wide haploid genetic screen identifies regulators of glutathione abundance and Ferroptosis sensitivity

Cells were then washed twice with mitochondrial assay solution (220 mM mannitol [Sigma-Aldrich, M4125], 70 mM sucrose [Sigma-Aldrich, S7903], 10 mM KH 2 PO 4 [VWR, 470302], 2 mM HEPES [Fisher Scientific, BP310], and 1 mM EGTA [VWR, 97062]) and then overlaid with 175 L of mitochondrial assay solution supplemented with 10 mM sodium pyruvate (Sigma-Aldrich, P5280), 1 mM malate (Sigma-Aldrich, M0875), 4 mM ADP (Sigma-Aldrich, A5285), and Seahorse Plasma Membrane Permeabilizer (Agilent, 102504-100)

[DOI] [PubMed] [Google Scholar] 28.Allocati N., Casalone E., Masulli M., Ceccarelli I., Carletti E., Parker M.W., Di Ilio C

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