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It has been observed, however, that fusion tags that are expressed in cells are cleaved by cellular proteases, which suggests that these tags can be modified by endogenous enzymes during experimental procedures
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[DOI] [PubMed] [Google Scholar] 333.Kobayashi E.H., Suzuki T., Funayama R., Nagashima T., Hayashi M., Sekine H., Tanaka N., Moriguchi T., Motohashi H., Nakayama K., et al
